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Kdp-dependent Kplus homeostasis of the halophilic archaeon Halobacterium salinarum

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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007121710
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DC ElementWertSprache
dc.contributor.advisorProf. Dr. Karlheinz Altendorf
dc.creatorStrahl, Henrik
dc.date.accessioned2010-01-30T14:48:17Z
dc.date.available2010-01-30T14:48:17Z
dc.date.issued2007-12-14T13:37:22Z
dc.date.submitted2007-12-14T13:37:22Z
dc.identifier.urihttps://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007121710-
dc.description.abstractHalobacteria balance high external osmolality by the accumulation of almost equimolar amounts of KCl. Thus, steady Kplus supply is a vital prerequisite for life of these extreme halophiles. So far, Kplus was supposed to enter the cell only passively by use of potential-driven uniporters. However, the genome of the extreme halophilic archaeon Halobacterium sp. NRC-1 comprises one single operon containing the genes kdpFABC coding for homologs of the bacterial ATP-driven Kplus uptake system KdpFABC, together with an additional ORF so far annotated as cat3. Deletion of the kdpFABCcat3 genes led to a reduced ability to grow under limiting Kplus concentrations, whereas real-time RT-PCR measurements revealed both high induction rates and a transcriptional regulation of the Kdp system dependent on external Kplus concentration and growth phase. The synthesis of the high-affinity KdpFABC complex enables H. salinarum to grow under extreme potassium-limiting conditions of down to 20 µM Kplus. These results provide the first experimental evidence of ATP-driven Kplus uptake in halobacteria. The current opinion that Kplus homeostasis of H. salinarum is solely mediated via membrane potential-driven Kplus uniporters is obviously only one aspect of a more complex system.eng
dc.language.isoeng
dc.subjectHalobacterium
dc.subjectpotassium uptake
dc.subjectKdpFABC
dc.subjectCat3
dc.subjectuniversal stress protein
dc.subject.ddc570 - Biowissenschaften, Biologieger
dc.titleKdp-dependent Kplus homeostasis of the halophilic archaeon Halobacterium salinarumeng
dc.typeDissertation oder Habilitation [doctoralThesis]-
thesis.locationOsnabrück-
thesis.institutionUniversität-
thesis.typeDissertation [thesis.doctoral]-
thesis.date2007-11-30T12:00:00Z-
elib.elibid734-
elib.marc.edtjost-
elib.dct.accessRightsa-
elib.dct.created2007-12-06T19:00:10Z-
elib.dct.modified2007-12-14T13:37:22Z-
dc.contributor.refereeProf. Dr. Felicitas Pfeifer
dc.subject.bk42.13 - Molekularbiologieger
dc.subject.bk42.30 - Mikrobiologieger
dc.subject.dnb32 - Biologieger
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