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Structural and functional analysis of the HOPS tethering complex at the yeast vacuole

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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2012081610269
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dc.contributor.advisorProf. Dr. Christian Ungermann
dc.creatorBröcker, Cornelia
dc.date.accessioned2012-08-16T09:39:04Z
dc.date.available2012-08-16T09:39:04Z
dc.date.issued2012-08-16T09:39:04Z
dc.identifier.urihttps://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2012081610269-
dc.description.abstractThe fusion of yeast vacuoles requires a Rab-GTPase (Ypt7), a tethering complex termed HOPS (homotypic fusion and vacuole protein sorting) and SNAREs. The HOPS complex consists of six subunits and is involved in the initial contact between late endosome (multi vesicular body) and the vacuole. The homologous CORVET complex shares four subunits with the HOPS complex and is required at the endosome. Upon overexpression, I was able to isolate the entire HOPS and stable subcomplexes consisting of two to six subunits. These subcomplexes might represent the core for the assembly, or may be transition intermediates. They could arise when the CORVET complex at the endosome matures into the HOPS complex at the vacuole. Using a structure-function approach, I analysed the HOPS structure via electron microscopy and its function via vacuole fusion assay.eng
dc.rightsNamensnennung-NichtKommerziell-KeineBearbeitung 3.0 Unported-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/-
dc.subjectBiochemistryeng
dc.subjectYeasteng
dc.subjectHOPS tethering complexeng
dc.subjectstructureeng
dc.subject.ddc500 - Naturwissenschaften
dc.subject.ddc570 - Biowissenschaften; Biologie
dc.titleStructural and functional analysis of the HOPS tethering complex at the yeast vacuoleeng
dc.typeDissertation oder Habilitation [doctoralThesis]-
thesis.locationOsnabrück-
thesis.institutionUniversität-
thesis.typeDissertation [thesis.doctoral]-
thesis.date2011-04-14-
dc.contributor.refereeapl. Prof. Dr. Siegfried Engelbrecht-Vandré
dc.subject.bk42.15 - Zellbiologie
vCard.ORGFB5
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