Photopatterning for probing protein-protein interactions in artificial model systems and live cells

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Titel: Photopatterning for probing protein-protein interactions in artificial model systems and live cells
Autor(en): Waichman, Sharon
Erstgutachter: Prof. Dr. Jacob Piehler
Zweitgutachter: Prof. Dr. Heinz-Jürgen Steinhoff
Zusammenfassung: Functional immobilization and lateral organization of proteins into micro- and nanopatterns is an important prerequisite for miniaturizing analytical and biotechnological devices. In this thesis I present novel and versatile approaches for high contrast surface micropatterning of proteins, artificial membranes and live cells based on maleimide photochemistry. The patterning strategy is carried-out on glass substrates exploiting a poly(ethylene glycol) PEG polymer layer as a compatible scaffold. The flexible PEG cushion prevents unspecific proteins attachment and cell adhesion to surfaces. The versatility of this method is demonstrated by means of different orthogonal chemistries using covalent- and affinity- based interactions for protein immobilization. Furthermore, using maleimide based alkyl-thiol chemistry, I utilized the patterning approach for capturing liposomes and proteoliposomes onto surfaces. Formation of fluid patterned polymer-supported membranes demonstrating lateral diffusion of lipids and proteins was confirmed by biophysical assays. A similar approach was used for micropatterning of transmembrane proteins in surface adhered live cells.
URL: https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2012101510347
Schlagworte: Surface chemistry; Protein-protein interactions; Photopatterning
Erscheinungsdatum: 15-Okt-2012
Enthalten in den Sammlungen:FB05 - E-Dissertationen

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