Molecular mechanism to target the endosomal Mon1-Ccz1 GEF complex to the pre-autophagosomal structure
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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201901241066
https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201901241066
Title: | Molecular mechanism to target the endosomal Mon1-Ccz1 GEF complex to the pre-autophagosomal structure |
Authors: | Jieqiong, Gao Langemeyer, Lars Kümmel, Daniel Reggiori, Fulvio Ungermann, Christian |
ORCID of the author: | https://orcid.org/0000-0003-2652-2686 |
Abstract: | During autophagy, a newly formed double membrane surrounds its cargo to generate the so-called autophagosome, which then fuses with a lysosome after closure. Previous work implicated that endosomal Rab7/Ypt7 associates to autophagosomes prior to their fusion with lysosomes. Here, we unravel how the Mon1-Ccz1 guanosine exchange factor (GEF) acting upstream of Ypt7 is specifically recruited to the pre-autophagosomal structure under starvation conditions. We find that Mon1-Ccz1 directly binds to Atg8, the yeast homolog of the members of the mammalian LC3 protein family. This requires at least one LIR motif in the Ccz1 C-terminus, which is essential for autophagy but not for endosomal transport. In agreement, only wild-type, but not LIR-mutated Mon1-Ccz1 promotes Atg8-dependent activation of Ypt7. Our data reveal how GEF targeting can specify the fate of a newly formed organelle and provide new insights into the regulation of autophagosome-lysosome fusion. |
Citations: | eLife 2018;7:e31145 |
URL: | https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-201901241066 |
Subject Keywords: | endosomal Mon1-Ccz1 GEF complex; pre-autophagosomal |
Issue Date: | 15-Feb-2018 |
License name: | Attribution 4.0 International |
License url: | http://creativecommons.org/licenses/by/4.0/ |
Type of publication: | Einzelbeitrag in einer wissenschaftlichen Zeitschrift [article] |
Appears in Collections: | FB05 - Hochschulschriften Open-Access-Publikationsfonds |
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eLIFE_7-e31145_2018_Gao.pdf | 5,46 MB | Adobe PDF | eLIFE_7-e31145_2018_Gao.pdf View/Open |
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