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Analysis of the protein composition of the spindle pole body during sporulation in Ashbya gossypii

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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202004202988
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dc.creatorWabner, Dario-
dc.creatorOverhageböck, Tom-
dc.creatorNordmann, Doris-
dc.creatorKronenberg, Julia-
dc.creatorKramer, Florian-
dc.creatorSchmitz, Hans-Peter-
dc.date.accessioned2020-04-20T08:58:52Z-
dc.date.available2020-04-20T08:58:52Z-
dc.date.issued2019-10-03-
dc.identifier.citationPLoS ONE 14(10): e0223374ger
dc.identifier.urihttps://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-202004202988-
dc.description.abstractThe spores of fungi come in a wide variety of forms and sizes, highly adapted to the route of dispersal and to survival under specific environmental conditions. The ascomycete Ashbya gossypii produces needle shaped spores with a length of 30 μm and a diameter of 1 μm. Formation of these spores relies on actin and actin regulatory proteins and is, therefore, distinct from the minor role that actin plays for spore formation in Saccharomyces cerevisiae. Using in vivo FRET-measurements of proteins labeled with fluorescent proteins, we investigate how the formin AgBnr2, a protein that promotes actin polymerization, integrates into the structure of the spindle pole body during sporulation. We also investigate the role of the A. gossypii homologs to the S. cerevisiae meiotic outer plaque proteins Spo74, Mpc54 and Ady4 for sporulation in A. gossypii. We found highest FRET of AgBnr2 with AgSpo74. Further experiments indicated that AgSpo74 is a main factor for targeting AgBnr2 to the spindle pole body. In agreement with these results, the Agspo74 deletion mutant produces no detectable spores, whereas deletion of Agmpc54 only has an effect on spore length and deletion of Agady4 has no detectable sporulation phenotype. Based on this study and in relation to previous results we suggest a model where AgBnr2 resides within an analogous structure to the meiotic outer plaque of S. cerevisiae. There it promotes formation of actin cables important for shaping the needle shaped spore structure.eng
dc.relationhttps://doi.org/10.1371/journal.pone.0223374ger
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectfungieng
dc.subjectFRET-measurementseng
dc.subjectpolymerizationger
dc.subject.ddc570 - Biowissenschaften, Biologieger
dc.titleAnalysis of the protein composition of the spindle pole body during sporulation in Ashbya gossypiieng
dc.typeEinzelbeitrag in einer wissenschaftlichen Zeitschrift [article]ger
orcid.creatorhttps://orcid.org/0000-0001-5449-4593-
dc.identifier.doi10.1371/journal.pone.0223374-
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